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eRev. [Major Version]1.01.0 8/9/2023
KrosFlo® KR2i Real-Time Process Management (RPM™) System
5. Remote Control On (“I” icon)
Note: The controls below are only accessible when the auxiliary pumps are in Internal control mode. When in External control mode,
the auxiliary pumps are controlled by input from the TFF system.
Table 15. KR Jr Tubing Specifications
L/S Precision pump tubing
L/S 13 L/S 14 L/S 16 L/S 25
Silicone tubing P/N ACTU-E13-25N ACTU-E14-25N ACTU-E16-25N N/A
Pharmapure tubing P/N ACTU-P13-25N ACTU-P14-25N ACTU-P16-25N ACTU-925-25N
Inside diameter (nominal) 0.8 mm (0.03”) 1.6 mm (0.06”) 3.1 mm (0.12”) 4.8 mm (0.19”)
Hose barb size 1.6 mm (1/16”) 1.6 mm (1/16”) 3.2 mm (⅛”) 4.8 mm (3/16”)
Flow rate range (mL/min) 0.005 – 12 0.014 –to 42 0.05 – 150 0.11 – 330
Maximum pressure 2.7 bar (40 psig) 2.4 bar (35 psig) 2.7 bar (40 psig) 2.4 bar (35 psig)
10.2.2 Auxiliary Pump Settings
In order for the TFF system to control the Auxiliary Pumps properly, input the Auxiliary Pump type and the tubing size being loaded
into the Auxiliary Pump pump heads.
10.3 Automatic Backpressure Valve
The KrosFlo® Automatic Backpressure Valve (ABV) controls a wide variety of pressure set-points during tangential flow filtration
processes when used in conjunction with the TFF System. The valve is designed to pinch flexible tubing to maintain the user-set
pressure. One ABV may be connected to the KR2i.
Figure 37. Automatic Backpressure Valve
10.3.1 ABV Installation
1. Plug in the valve serial port to the serial connector labeled "Valve" on the Octopus Cable. The valve is powered through the
octopus connector.
2. Place the tubing through the plunger mechanism by lifting up on the body of the valve and fitting the tubing between the
metal bar and the white plastic plunger. The body of the valve can then be turned 180° to close the tubing opening by using
the longer stainless steel rods or let the tubing opening remain accessible with the shorter rods.
10.3.2 ABV Settings
Auto Mode Menu
a. Mode: Can be set to Auto or Manual-- if set to Manual, settings will switch to Manual Mode Menu
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