-30-
(2) Modification of non 100/0 microscope
Some microscopes, typically Olympus BX50 series, have a slider (switching between
eyepiece and camera port) other than 100/0 or 0/100, such as 20/80. If the switching
slider was set at a non-100/0 position, part of the reflected laser beam will reach into
the microscope eyepiece. Never use such non-100/0 position for your own safety. It
is highly recommended to seal such non-100/0 slider windows with black tape so as
to prevent accidental laser exposure. Figure 3-2 shows examples of where to seal the
20/80 windows of the switching slide of the Olympus BX50 microscope. Please make
sure both windows (eyepiece side and camera port side) are sealed. Furthermore, it
is strongly recommended that you should always shut CSU-X1 shutter when you are
not observing confocal images, so that you can avoid accidental laser exposure.
Figure 3-2 Where to seal the 20/80 windows of the switching slider of the Olympus
BX50 microscope
When you switch the light path between confocal and non-confocal observation,
(epifluorescence, transmitted light image, etc.) reflected laser beam could reach into
the microscope eyepiece at the moment of switching due to the reflection at the edge
of switching prism.
Never look into the microscope eyepiece when you change the light path, and always
shut the CSU-X1 shutter when you switch the light path, or turn off the laser when
you switch the light path.
(3) Laser warning labels
Please attach laser warning labels on your microscope as indicated in Figure 1-3.
100/0
80/20
0/100
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