Chapter 3 Individual Operations
118
Individual
Operations
16
Epi-fluorescence Microscopy
Epi-fluorescence microscopy used for the observation of a fluorescence image requires a brighter illuminator including an
optical element such as a filter or mercury lamp.
In Ni-E, an epi-fluorescence cube turret (motorized, intelligent, or manual) to which a filter cube is attached, HG
precentered fiber illuminator (motorized or manual) which uses mercury lamp as the illuminator, and epi-fluorescence
attachment equipped with an ND filter, aperture diaphragm, and field diaphragm are used.
WARNING
The light source used with the epi-fluorescence attachment (mercury lamp) requires special care during handling
because of its characteristics. Make sure you are familiar with and adhere to all warnings and cautions described at
the beginning of this instruction manual.
16.1
Epi-fluorescence Cube Turret Operation (Switching Excitation Methods)
The epi-fluorescence cube turret has six slots. Attach the filter cube to a slot and bring it into the optical path. For
bright-field microscopy, be sure to set Address 1 to empty [OPEN] and bring it into the optical path.
Motorized epi-fluorescence cube turret operation
Use a FL CUBE CW/CCW button to switch the epi-fluorescence
cube turret. The turret rotates to the adjacent address each time
the button is pressed.
Attachment information display for the filter cube is disabled by
factory default.
Set the filter cube attachment information to the
epi-fluorescence cube turret and switch it. The information on
the filter cube on the optical path will be displayed on the display
panel.
In addition, the front slot cover for a filter cube replacement has
a window for inserting a filter cube nameplate. It is also useful if
you keep inserting the nameplate into the window at the address
to which the filter cube was attached.
Set the attachment information from DS-L3. (See “23 Operation
on DS-L3” - “23.1 Setting Up the Microscope - (1) Setting
Optical Elements Installation Information” for details.)
For information on attaching filter cubes to the turret, see
Chapter 1 “3 Assembly - 10 Attach the epi-fluorescence cube
turret and epi-fluorescence attachment - ■ Attaching the filter
cube” in the “Assembly/Maintenance” instruction manual.
Operation on DS-L3 (See “23.2 Controlling the Microscope”.)
ND
8
ND
32
OUT
IN
NCB
11
Switching of the epi-fluorescence cube turret
PA__2x____Z:_____0.000um
UV-2B
'
C __=_ND_ 1
'
C __EPI:C
1
2
A
λ
2
3
4
5
6
50
60
70
Inserting the nameplate into the address window
Filter cube nameplate
window
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