Chapter 3 Individual Operations
128
Individual
Operations
■ Objectives
The excitation balancer should be used in combination with the following objectives. Use of other objectives may result
in unevenness in the field of view.
Plan fluor 40x/0.75 40xH/1.3 100xH/1.3
S fluor 40x/0.9 40xH/1.3 100xH/1.3
Plan apochromat 40x/0.95 60xH/1.4 100xH/1.4
16.7
Switching the Excitation Filter (Motorized Excitation Filter Wheel)
By attaching a motorized excitation filter wheel between the
epi-fluorescence attachment and the HG precentered fiber
illuminator, switching of the excitation filters can be motorized.
Up to 8 filters can be attached to the wheel.
A reflective ND filter can be attached to each excitation filter.
See Chapter 1 “3 Assembly Method - 13 Attach the motorized
excitation filter wheel” in the “Assembly/Maintenance” instruction
manual for attaching the excitation filter.
Configure the information on attaching the excitation filter to the
wheel on DS-L3. (See “23 Operation on DS-L3” - “23.1 Setting
Up the Microscope - (1) Setting Optical Elements Installation
Information” for details.)
The wheel is rotated by the FUNCTION button on the Ni-E
microscope main body. Press FUNCTION button 3 or 4 to rotate
the wheel counterclockwise or clockwise and change the wheel
address one by one. An excitation filter indicated on the display
panel on the front of the Ni-E microscope is brought into the
optical path.
Operation on DS-L3 (See “23.2 Controlling the Microscope”.)
ND4
ND8
ND16
Switching of excitation filter
BA400_____DAPI_ -2
'
C _____
EX330-380_ UV-2B
'
C __EPI:C
Motorized excitation
filter wheel
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