Nikon Eclipse LV100DA-U User Manual

30
2
Focus on the specimen with the bright-field microscopy under the diascopic illumination.
(See Pages 26 to 27.)
* If the specimen is transparent, it may be difficult for the bright-field microscopy to focus on the
specimen. To observe a transparent specimen, switch the microscopy to the phase contrast
microscopy according to the following procedure and then focus on the specimen.
3
Set up the microscope for the phase contrast
microscopy under the diascopic illumination.
1 Press the OBJ. switch on the operation panel and
locate the Ph objective into the optical path.
(See Page 43.)
Check the Ph code indicated on the body part of
the Ph objective.
2 Set the condenser turret of the universal
condenser to the “Ph1”, the “Ph2”, or the “Ph3”
position, which is corresponding to the
objective’s Ph code, and locate the Ph annular
diaphragm into the optical path. (See Page 55.)
3 Operate the DIA brightness switch on the
operation panel to adjust the brightness of the
diascopic illumination. (See Page 42.)
4 Adjust the brightness with ND filters. (See Page 44.)
5 Fully open the field diaphragm of the condenser.
(See Page 54.)
6 Center the Ph annular diaphragm.
For the centering procedure, see the instruction manual for the LV-CUD.
7 Adjust the size of the field diaphragm so that the field diaphragm image circumscribes or
inscribes the field of view. (See Page 54.)
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BF DF FL1 FL2
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OUT
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DIA
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Operation
panel
OBJ. switch
DIA brightness switch
Note on the phase contrast microscopy under the Diascopic illumination
The view of a phase contrast image depends on the phase contrast characteristics or the shape of the
specimen and the characteristic of the objective. For details about the phase contrast microscopy, refer to
the LV-CUD Instruction manual.
10
Phase Contrast Microscopy under the Dia Illumination
1
Attach the accessories required for the phase contrast microscopy under the dia illumination.
The following accessories must be attached to perform the phase contrast microscopy under the dia
illumination.
• Universal condenser (dry) (See Page 78 and the LV-CUD instruction manual.)
• Ph objective (See Page 93.)
• Ph annular diaphragm (attached inside the universal condenser) (See the LV-CUD instruction manual.)
Attach the Ph annular diaphragm corresponding to the objective's Ph code into the universal
condenser. For details, see the LV-CUD instruction manual.
Note that the “Ph” symbol in the device name stands for “phase contrast.”

Nikon Eclipse LV100DA-U Specifications

General

Brand	Nikon
Model	Eclipse LV100DA-U
Category	Microscope
Language	English