49
III. Operation of Each Part
10
Diopter Adjustment
Diopter adjustment compensates for the difference in visual acuity between the right and left eyes.
This adjustment improves binocular observation and minimizes focal deviation when switching
objectives. Make sure to adjust the diopter adjustment rings on both eyepieces.
1 Rotate the diopter adjustment rings of the eyepieces and align their engraved lines with the
edges of the outer tubes of the eyepieces (They are the standard positions for the diopter rings.)
2 Follow the procedure described in Pages 15 and 16 for the bright-field microscopy under the
episcopic illumination (or Pages 26 and 27 for the bright-field microscopy under the
diascopic illumination), and focus the specimen with the 10x objective.
3 Locate the 50x objective into the optical path. Rotate the coarse/fine focus knobs to focus on
the specimen.S
4 Locate the 5x or 10x objective into the optical path.
5 Focus on the specimen with the diopter adjustment rings not with the coarse/fine focus knobs.
Look through the left eyepiece with your left eye and the right eyepiece with your right eye
to focus on the specimen.
6 Repeat the steps 3 to 5 using the 50x objective and the 5x or 10x objective, until the focus
shift is eliminated even when the lens magnification is changed.
11
Interpupillary Distance Adjustment
Before performing the interpupillary distance
adjustment, follow the procedure described in
Pages 15 and 16 for the bright-field microscopy
under the episcopic illumination (or Pages 26 and
27 for the bright-field microscopy under the
diascopic illumination), and focus on the specimen
with the 10x objective.
Adjust the interpupillary distance so that the
viewfields for both eyes come together.
This will facilitate the observation with both eyes.
The binocular part has a scale for interpupillary
distance. It is recommended to memorize or record
your interpupillary distance, so that the distance
between the eyepieces can be adjusted with ease
next time.
U
E
PI2
A
US
B
F
.
STOP
J
A
P
A
N
BF DF FL1 FL2
FL1
FL2
1
00
2
0
0
1
0
0
IN
OUT
LV
-
T
T2
Engraved
line
Diopter adjustment standard position
Diopter
adjustment
ring
Positioning
grooves
Outer tube
edge
UEPI2A
USB
F
.
STOP
J
APAN
BF DF FL1 FL2
FL1
FL2
10
0
20
0
100
IN
OUT
LV
-
T
T
2
Converge until the right and left
view fields coincide.