Nikon Eclipse LV100DA-U User Manual

32
11
Differential Interference Contrast Microscopy under the Dia Illumination
1
Attach the accessories required for the DIC microscopy under the dia illumination.
The following accessories must be attached to perform the DIC microscopy under the dia illumination.
• Universal condenser (dry) (See Page 78 and the LV-CUD instruction manual.)
• Rotatable polarizer for the diascopic illumination (See Page 94.)
• Analyzer (See Page 83.)
• DIC objective for biologic microscopes (See Page 93.)
• Condenser DIC prism (attached inside the universal condenser)
(See Page 66 and the LV-CUD instruction manual.)
• Objective DIC prism (attached to the nosepiece) (See Page 81.)
• Lambda plate holder (for the sensitive color microscopy) (See Page 80.)
* Set up the microscope according to the “LVSetup” and set information of objectives correctly.
2
Focus on the specimen with the bright-field microscopy under the diascopic illumination.
(See Pages 26 to 27.)
3
Set the microscope for the DIC microscopy under the diascopic illumination.
1 Push in the analyzer slider to locate the analyzer
into the optical path. (See Page 60.)
2 Attach the rotatable polarizer for the diascopic
illumination and rotate the upper part of the
polarizer to make a crossed Nicols position.
(See Page 59.)
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OBJ. switch
DIA brightness switch
3 Press the OBJ. switch on the operation panel and
locate the objective for the DIC microscopy into
the optical path. (See Page 43.)
4 Attach the objective DIC prism slider to the
nosepiece to locate the objective DIC prism into the
optical path. (See Page 66.)
5 Set the condenser turret to the “N1,” “N2,” or “NR”
position to locate the condenser DIC prism suitable
for the objective into the optical path. (See Page 55.)
6 Operate the DIA brightness switch on the
operation panel to adjust the brightness of the
diascopic illumination. (See Page 42.)
7 Adjust the brightness with ND filters. (See Page 44.)
The Senarmont method is used for the DIC microscopy under the dia illumination.

Nikon Eclipse LV100DA-U Specifications

General

Brand	Nikon
Model	Eclipse LV100DA-U
Category	Microscope
Language	English