Chapter 1 Microscopy Procedures
3
Chap. 1-1
Microscopy Procedures
Bright-field Microscopy
1.2
Bright-field Microscopy Procedure
1. Turn on the power.
2. (If using the toggle function)
Configure the toggle pattern.
3. Switch to operation mode (left side).
4. Turn ON the dia-illumination LED.
5. Lower condenser slightly from
uppermost position.
6. Fully open the field and aperture
diaphragms.
7. Bring the 10x objective into the optical
path.
8. Bring a specimen into the optical path.
9. Focus on the specimen.
10. Adjust the diopter.
11. Adjust the interpupillary distance.
12. Focus and center the condenser.
13. Bring the desired objective into the
optical path.
14. Adjust the aperture diaphragm.
15. Focus on the specimen.
16. Circumscribe the field diaphragm to
the field of view.
17. View the specimen.
18. Turn off the power.
OFF 12
TOGGLE
PATTE RN
MEMORY
CLAMP
TORQU E
0.1
0.3
0.4
0.5
0.2
0.9
0.8
Preparation for microscopy
1
Turn on the power.
Turn on the power switch (press to the “|” position) for the
microscope. (The power LED on the front of the main
body will light up.)
Power switch ON
POWER
LED Illuminated
ON
8
4
10
7
13
11
15
9
12
5
6
16
12
14
6
17
TORQUE
P
O
WER
0
90
80
70
60
50
40
30
20
10
7
13
3
2
1
18