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Eclipse Ci-E
Nikon Eclipse Ci-E User Manual
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67
Assembly
3
Chap. 3
Assembly
1
ECLIPSE Ci-E Syste
m Configuration
0.2
0.4
0.6
0.8
1.0
1.2
1.4
Achr-Apl N.A=1.4
Eyelevel rise
r
A
nalyzer unit
Filter cube
CI-FL
or D-FL Epi-fluoresc
ence
attachm
ent
HG precent
ered
fiber illuminator
Ergonomic tub
e
Binocular t
ube
T
rinocular tub
e
DSC port
Centering
telescope
CFI
e
y
e
p
iece
TV adapte
r
Ci-E main bod
y
S
pacer for the
nose
p
iece
S
tage
Condense
r
Filte
r
cassette
holder
Polarize
r
unit
ENG mount cam
era
C mount camera
photomicrogra
phy
device
DS-L4
Microsco
pe came
ra
control unit
Camera head
Camera trigge
r
cable
Camera cable
Remote
control
pad
A
nalyzer slide
r
CFI objectiv
e
USB
cable
USB cable
78
80
Table of Contents
Introduction
3
Contents of the Manual
4
Symbols Used in this Manual
4
Table of Chapters
5
Table of Contents
6
Safety Precautions
9
WARNING and CAUTION Symbols
9
Meaning of Symbols Used on the Product
9
Warning
9
Caution
9
Notes on Handling the Product
12
Microscopy Procedures
13
Bright-Field Microscopy
13
System Configuration and Controls
13
Bright-Field Microscopy Procedure
15
Phase Contrast Microscopy
23
System Components and Controls
23
Phase Contrast Microscopy Procedure
25
Simple Polarizing Microscopy
30
System Configuration and Controls
30
Simple Polarizing Microscopy Procedure
32
Sensitive Polarization Microscopy
37
System Configuration and Controls
37
Sensitive Polarization Microscopy Procedure
39
Epi-Fluorescence Microscopy
44
System Configuration and Controls
44
Epi-Fluorescence Microscopy Procedure
46
Individual Operations
51
Adjusting the Brightness of a Diascopic Image
51
Adjustment with the Dia-Illumination Brightness Control Knob
51
Adjustment with ND Filters
51
Focusing on the Specimen (Vertical Stage Movement)
52
Focus Knob Rotation and Stage Movement
53
Number of Focus Knob Turns and Distance of Stage Travel
53
Adjusting the Rotating Torque of the Coarse Focus Knob
54
Refocusing
54
Position Exchange of the Fine Focus Knob
55
Bringing the Specimen into the Optical Path (Horizontal Stage Movement)
56
Knob Rotation and Stage Movement
56
Adjusting the Knob Heights
56
Adjusting the Knob Rotation Torque
56
Adjusting the Diopter
57
Focusing and Centering the Condenser
58
Adjusting the Aperture Diaphragm
59
Adjusting the Aperture Diaphragm Using the Condenser Scale
59
Adjusting the Aperture Diaphragm Using the Centering Telescope
59
Selecting a Condenser
60
Adjusting the Field Diaphragm
60
Switching the Optical Path of the Tube
61
Light Distribution
61
Disabling the Clicking of the Optical Path Switching
61
Adjusting the Binocular Section
62
Adjusting with the Binocular Section of the Tube
62
Using the Eyelevel Riser
62
Using at Lowered Position of Stage (Spacer for Nosepiece)
62
Switching Objectives
63
Basic Operation
63
Switching between Two Objectives (Toggle)
64
Configuring the Motorized Functions
66
Configuring the Motorized Swing-Out Condenser
66
Changing the Initial Brightness of Dia-Illumination LED
67
Reverse Rotation Lock (Rotation from Address 1 to 6)
68
Oil Immersion
69
Water Immersion
70
Tips for Phase Contrast Microscopy
70
Tips for Epi-Fluorescence Microscopy
72
Switching Excitation Methods
73
Selecting Filters
74
Capturing Images
76
Photomicroscopy
76
Tips on Microscope Settings for Photomicroscopy
77
Assembly
79
ECLIPSE CI-E System Configuration
79
Assembly for Bright-Field Microscopy
80
Assembly for Phase Contrast Microscopy
85
Assembly for the Simple Polarizing Microscopy
85
Assembly for Sensitive Polarization Microscopy
86
Assembly for Epi-Fluorescence Microscopy
87
Attaching a Camera
89
Troubleshooting
91
Optical System and Operation
91
General
91
Epi-Fluorescence Microscopy
95
Phase Contrast Microscopy
96
Electrical System
97
General
97
Epi-Fluorescence Microscopy
98
Maintenance and Storage
99
Cleaning
99
Cleaning Lenses
99
Cleaning Parts Other than the Lens
99
Cleaning Immersion Oil
100
Decontaminating the Product
100
Storage
100
Regular Inspections (Charged)
100
Specifications and Safety Standards
101
Microscopy (Principles)
101
Performance Properties
101
Physical Properties
102
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Nikon Eclipse Ci-E Specifications
General
Brand
Nikon
Model
Eclipse Ci-E
Category
Microscope
Language
English
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