Nikon Eclipse Ci-E User Manual

Chapter 3 Assembly
75
Chap. 3
Assembly
6
Assembly for Epi-fluorescence Microscopy
Follow the procedure described in Section 2 “Assembly for Bright-field Microscopy” to perform assembly.
Note the following:
(For details on attaching a D-FL Epi-fluorescence attachment, refer to the instruction manual provided with the D-FL
Epi-fluorescence attachment.)
■ Attaching the epi-fluorescence attachment
Attach the CI-FL Epi-fluorescence attachment to the microscope arm prior to the step 4, “Attach the tube.”
(1) Place the epi-fluorescence attachment on the
microscope arm.
(2) Secure it by tightening the clamp screw on the arm and
two fixing bolts at the rear of the epi-fluorescence
attachment. Use a hex wrench provided with the
epi-fluorescence attachment to tighten the fixing bolts.
(3) Attach the HG adapter of the mercury lamp illuminator
to be used to the bayonet mount on the back. (For
detailed information, refer to the instruction manual
provided with the mercury lamp illuminator.)
(4) Place the tube on the epi-fluorescence attachment and
tighten the tube clamp screws of the epi-fluorescence
attachment using the tool provided with the microscope
to secure the tube.
A 1
-
2
-
3
-
4
B 1
-
2 / 3
-
4
C 1
-
2
-
3
-
4
1
1
4
2 3 4
CUBE
Attaching the epi-fluorescence attachment
■ Attaching a filter cube
WARNING
Always turn off the mercury lamp before inserting or removing a filter cube.
(1) Remove the filter cube replacement cover on the left o
f
the epi-fluorescence attachment.
(2) Insert a filter cube.
The filter cubes listed below cannot be inserted directly
into the filter bay of the epi-fluorescence attachment.
Remove an internal spacer or reverse the spacer
before insertion.
<Filter cubes requiring spacer removal>
 UV-2A
 UV-2B
<Filter cubes requiring spacer reversal>
 DAPI
 FITC
 GFP-L
 GFP-B
 TRITC
 Tx-Red
A1
-
2
-
3
-
4
B 1
-
2 / 3
-
4
C 1
-
2
-
3
-
4
A
UV-2A
EX 330-380
DM 400
BA 420
ND4
ND8
ND16
A1
-
2
-
3
-
4
B1
-
2 / 3
-
4
C1
-
2
-
3
-
4
Attaching a filter cube
Epi-fluorescence
attachment
clamp screw
Epi-fluores-
cence
attachment
HG adapte
r
Epi-fl attachment clamp bolts
(use a tool)
Filter cube
Tube clamp screw
(use a tool)
Filter cube
re
p
lacement cove
r

Nikon Eclipse Ci-E Specifications

General

Brand	Nikon
Model	Eclipse Ci-E
Category	Microscope
Language	English