Chapter 4 Troubleshooting
84
Chap. 4
Troubleshooting
1.3
Phase Contrast Microscopy
Problem Cause Measure
Poor contrast.
The Ph annular diaphragm of the
condenser does not match the phase plate
image of the objective.
Adjust so that they match.
(→Chapter 1, “2.2 Phase Contrast Microscopy
Procedure - 15 Center the Ph annular diaphragm”.)
The Ph annular diaphragm of the
condenser and the objective selected do
not match.
Put the Ph annular diaphragm with the same
Ph code as the objective into the optical path.
(→Chapter 1, “2.2 Phase Contrast Microscopy
Procedure - 17 Adjust the Ph annular diaphragm in
the condenser with the Ph objective to be used”.)
The phase contrast of the specimen is too
large.
Change the mounting agent or thickness of
the sample when preparing the specimen.
(→Chapter 2, “16 Tips for Phase Contrast
Microscopy”.)
The type of the Ph objective is not suitable
for phase contrast of the specimen.
Use a Ph objective suitable for the specimen.
(→Chapter 2, “16 Tips for Phase Contrast
Microscopy”.)
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