Chapter 1 Microscopy Procedures
32
Microscopy Procedures Epi-fluorescence Microscopy
Chap. 1-5
5
Epi-fluorescence Microscopy
5.1
System Configuration and Controls
This section explains an example system configuration and the controls required for an epi-fluorescence microscopy using
the ECLIPSE Ci-E.
Names of components are denoted in the following manner: [CI-FL epi-fluorescence attachment].
INTENSILIGHT
C
-
HGFI
LAMP
ND
1
2
4
32
16
8
SHUTTER
POWER
RUN TIME hrs.
CLAMP
TORQUE
0.1
0.3
0.4
0.5
0.2
0.9
0.8
A1
-
2
-
3
-
4
B 1
-
2 / 3
-
4
C1
-
2
-
3
-
4
1
1
4
2 3 4
CUBE
DSC
MODEL ECLIPSE Ci-E
USB REMOTE FOOT SWITCH
NIKON CORPORATION
TOKYO, JAPAN
100–240V~
1.0A
50/60Hz
MADE IN CHINA
This device complies with Part 15 of the FCC
Rules. Operation is subject to the following two
conditions:
(1) this device may not cause harmful interference,
and (2) this device must accept any interference
received, including interference that may cause
undesired operation.
This Class A digital apparatus complies with
Canadian ICES-003.
Cet appareil numérique de la classe A est
confirme à la norme NMB-003 du Canada.
930001
4N75
INSPECTION
EQUIPMENT
70
60
50
40
30
20
10
0
90
80
knob
X knob
[Eyepiece]
[Objective]
[Stage]
[Tube]
[Nosepiece]
[Main body]
Diopter
adjustment
ring
[CI-FL epi-fluorescence
attachment
[Condenser]
Coarse focus clamp ring
Optical path
switching lever
perture diaphragm leve
Power switch
Nosepiece forward
rotation button
Condenser focus knob
Field diaphragm leve
Filter cube switching knob
Shutter open/close leve
Filter cube motion
restricting lever
[HG adapter]
[HG fiber]
[HG precentered fiber illuminator]
(For details, see the instruction manual for
the HG precentered fiber illuminator.)
Coarse focus
knob
Fine focus
knob
Capture button
Field diaphragm dial
Nosepiece reverse
rotation button
UE
P
O
WER
Condenser focus knob
Condenser centering
screw
Coarse focus torque
adjustment knob
Swing-out condenser connecto
Fine focus knob
Moving claw of the
s
ecimen holde
Filter cube replacement
cover
Light shielding plate
ND filter slide
Nosepiece reverse
rotation button
Nosepiece forward
rotation button
Coarse focus knob
Grip
Input voltage label
DSC connecto
Lamphouse
REMOTE Connecto
USB connecto
AC inle
FOOT SWITCH
connecto
Dia-illumination LED
ON/OFF switch
Dia-illumination
brightness control
knob