Chapter 7 Specific Operations
81
13
Using Epi-Fluorescence Illumination
Epi-fluorescence microscopy used for observing fluorescence
images requires an optical element such as a fluorescence filter,
and a brighter light source including mercury lamp.
In this microscope system, state detection or motorized-type
epi-fluorescence illumination is available. With a filter cube
attached to the device, the filter cube is brought into the optical
path by the rotation of the turret, resulting in the generation of
epi-fluorescence illumination. The light source is a precentered
high-intensity mercury lamp.
Illumination led from the light source to the device by the HG fiber
passes through a fluorescence filter and emits excitation light to
the sample so that fluorescent images can be observed.
See Chapter 8, “9 Epi-Fluorescence Attachment” and select the
appropriate device based on the objective of microscopy.
• P2-EFLI Epi Fluorescence Attachment
• P2-EFLM Motorized Epi Fluorescence Attachment
For details on adjusting the light sources such as brightness
adjustment, refer to the instruction manuals of the light sources.
Epi-fluorescence illumination with the epi
fluorescence attachment
WARNING
The light source used with the epi fluorescence attachment (HG precentered fiber illuminator) requires special care during
handling because of its characteristics. Make sure you are familiar with and observe all warnings and cautions described at the
beginning of this instruction manual.
13.1
Switching Filter Cubes
The turret of the epi fluorescence attachment is rotated to switch the four filter cubes attached to the turret.
[Turret rotation operation]
P2-EFLI Epi Fluorescence Attachment: Filter cube switching knob of the device
P2-EFLM Motorized Epi Fluorescence Attachment: Filter cube switching buttons of the remote controller
BINO
PHOTO
2
1
1 2 3 4
DISPLAYDISPLAY
BRIGHTNESSBRIGHTNESS
ESCAPEESCAPE
ALZALZ
−+
LIMITLIMIT
ZOOMZOOM
STEPSTEP
Z-RESETZ-RESET
Z-SPEEDZ-SPEED ZOOM STEPZOOM STEP
CoarseCoarse
FL CubeFL Cube
ON/OFFON/OFF ON/OFFON/OFF
ON/OFFON/OFF ON/OFFON/OFF
FineFine Ex FineEx Fine 1x1x 0.5x0.5x 0.1x0.1x
+
−
Switching filter cubes
On the P2-EFLI Epi Fluorescence Attachment, turn the filter cube switching knob to assign to the indicator the turret address
(FL1 to FL4) of the filter cube to be placed into the optical path.
On the remote control, press the filter cube switching buttons (FL1 to FL4) to specify the turret address of the filter cube to be
placed into the optical path. The LED of the pressed button lights so that the turret address on the optical path can be
identified. The motorized epi fluorescence attachment has a turret address view window on the right front so that the turret
address on the optical path can be identified.
Filter cube switching
knob
Filter cube switching
buttons
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