Chapter 7 Specific Operations
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15.2
Tips on Microscope Settings for Photomicroscopy
Confirming the photomicrographic range
The image on the monitor represents the photomicrographic range.
Adjusting to eliminate extraneous light
Cover the eyepiece with a piece of cloth or similar item.
Protecting fluorescent images from decoloration
The fluorescence of specimens may fade during exposure. To prevent this, do the following:
• Adjusting the excitation light
Excessively bright excitation light will accelerate the decoloration of the sample making it more difficult to obtain
suitable fluorescent images. Use the ND filter of the light source to perform adjustment.
• Sample
Photomicrography of the color-fading portion requires a prolonged exposure time resulting in poor color reproduction
and low-quality images. Move the sample so that the image of the portion that has not been exposed to excitation light
can be captured. To obtain the best results, use the diascopic illumination method to select a specimen section for
photomicrography, and then switch to the fluorescent method to capture the image.
• Using the shutter
When not performing observation, close the shutter.
Adjusting the brightness of the image on the monitor
When observing images on the monitor via a DS camera, the brightness can be changed also by making an adjustment
on the DS camera side, for example, by changing the display mode, exposure mode and photometry mode, by
compensating the exposure or by adjusting the image level.
For details, refer to the instruction manual for the DS-L4.
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