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YL Instruments YL9100 HPLC User Manual

YL Instruments YL9100 HPLC
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YL9111 Binary Pump
Pulsation by compressibility and delay Compensation of pulsation
[Fig. 2] Delivery mechanism of YL9111 Binary pump
2-4. Gradient Curve
Solvent A and solvent B are mixed with flow rate Fb,
Mixed solvent flows to the column with flow rate Fab,
Solvent volume V of solvent A vs Concentration of solvent B
C(%) = 100 x {1 - (F
ab
-F
b
)/V x t}
(Fb/Fab-Fb)
The solvent ratio difference between present row and next row increases or decreases as a exponential
curve. In general, the gradient elution is used for separation of duplicated peaks. YL9111 Binary pump
has a exponential curve program, but use gradient elution by linear curve when it operates with YLClarity
software.
[Fig. 4] Gradient curve
6

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YL Instruments YL9100 HPLC Specifications

General IconGeneral
BrandYL Instruments
ModelYL9100 HPLC
CategoryLaboratory Equipment
LanguageEnglish