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YL Instruments YL9100 HPLC User Manual

YL Instruments YL9100 HPLC
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YL9101 Vacuum Degasser
Chapter 3. Operation
There are four LEDs in front of YL9101 Vacuum Degasser.
POWER LED turns ON if main power turns on
VACUUM LED turns ON during vacuuming
STATUS LED turns ON if there is error during vacuum degassing
3-1. Degassing of the solvent
Power on YL9101 Vacuum Degasser by pressing power switch, which is located in the front. When you
power on, check if pump is stopped, otherwise stop pump before turning on YL9101 Vacuum Degasser.
YL9101 Vacuum Degasser will start immediately to degas solvent. If normally operational, green LED
(VACUUM) located far left is on.
3-2. Operating of solvent delivery pump
Set mixing rate constant for all solvents and start pump at flow rate of 1.0 ml/min. Air bubble will be
coming when start. To remove the bubble, open the prime/purge valve and pull out air bubble by syringe
slowly until the bubbles are removed. After remove, close the valve for normal use of pump.
3-3. Replacing solvents
When necessary to replace solvents during analysis, stop pump and replace solvents and re-start
pump. But do not turn off YL9101 Vacuum Degasser. If you want to change solvent filled inside if YL9101
Vacuum Degasser completely, set mixing rate at 100% for the solvent, pull out more than 15 ml solvent
by syringe or start up pump and wait until solvent is completely changed.
3-4. Keeping method after use
If you do not use YL9101 Vacuum Degasser more than 1 day, use ultra pure water and flow into
vacuum degasser and rinse with alcohol completely and keep it. Specially when use with buffering
solution, be sure to rinse YL9101 Vacuum Degasser, otherwise precipitation can be occurred. In the case
YL9101 Vacuum Degasser is kept lower than 0, dry completely after rinse.
4

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YL Instruments YL9100 HPLC Specifications

General IconGeneral
BrandYL Instruments
ModelYL9100 HPLC
CategoryLaboratory Equipment
LanguageEnglish