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Troubleshooting
5
Misuse of the product may result in poor product performance, even if the product is properly functional. If
you experience any of the following problems, check the following table for possible causes before
requesting service.
If the problem is not listed below, or if the problem cannot be resolved by the suggested countermeasure,
unplug the power cord and contact Nikon.
5.1 Image Viewing
Problem Possible cause Countermeasure
Incorrectly attached part. Attach all parts correctly.
Movable part in inappropriate position.
The following parts must be moved to
click-stop positions: optical path selector
knob, nosepiece, filter sliders, condenser
turret, and Bertrand lens in/out lever.
Interference of the concentric ring on the
stage with the optical path.
Move the specimen.
Field diaphragm image not focused on
focal plane of specimen.
Focus and center the condenser
correctly.
Opening of the field diaphragm too
narrow.
Open the field diaphragm to be slightly
larger than the field of view.
Vignetted field of view.
No visible image.
Uneven brightness in field
of view.
Image too dark.
Dirt or dust on the lens or other optical
element, or on the culture vessel.
Clean the optical elements.
Use a clean culture vessel.
Dirt or dust on the lens or other optical
element, or on the culture vessel.
Clean the optical elements.
Use a clean culture vessel.
Dirt or dust is seen in
the field of view.
Field diaphragm image not focused on
focal plane of specimen.
Focus and center the condenser
correctly.
Dirt or dust on the lens or other optical
element, or on the culture vessel.
Clean the optical elements.
Use a clean culture vessel.
Objective correction ring not matched to
culture vessel’s bottom plate thickness.
Use an appropriate correction ring.
Culture vessel’s bottom plate thickness is
out of the objective’s correction range.
Use a culture vessel with bottom plate
thickness within the correction range.
Poor image quality.
Poor contrast or
resolution.
Field diaphragm image not focused on
focal plane of specimen.
Focus and center the condenser
correctly.
Bright-field objective used. Use a phase contrast objective.
Condenser annular diaphragm not in the
optical path.
Move an annular diaphragm with the
same Ph code as the phase contrast
objective into the optical path.
Annular diaphragm not centered. Center the annular diaphragm.
Phase contrast effect
cannot be obtained in
phase contrast
microscopy.
Aperture diaphragm of system condenser
not fully open.
Fully open the diaphragm.
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