Chapter 2 Microscopy
2.2 Bright-Field (BF) Microscopy
36
10
Observe the specimen.
Coarse
Fine
ExFine
Obj.
ON
OFF
6V30W
M
AX.
12V
100W
L80
EYE
DISPLAY
MEMORY
PFS
ON
RECALL
Z
-
RESET
BRIGHTNESS
R100
L100
FOCUS
B
ExFine
Fine
Coarse
Epi Shutter
FL Block
Refocus
Escape
PFS
OFFSET
OUT
D
I
C
HRO
I
C
M
IRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z
-
RESET
1X
1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
Aperture diaphragm adjustment
70~80
100
Adjust the size of the aperture diaphragm image to
70-80% the size of the objective pupil plane.
1. Move an objective with the desired
magnification into the optical path by
pressing the Obj. switch.
2. Adjust the size of the aperture diaphragm to
“70-80% the size of the NA of the objective”
by moving the aperture diaphragm
open/close lever on the system condenser.
Move the Bertrand lens into the optical path by
moving the Bertrand lens in/out lever on the
eyepiece tube to position “B”. This will allow you
to observe the objective pupil plane and the
aperture diaphragm image. Adjust the focus by
rotating the Bertrand lens focusing knob on the
right side of the eyepiece tube, and then adjust
the size of the aperture diaphragm image to
70-80% the size of the objective pupil plane.
When done, move the Bertrand lens out of the
optical path by moving the operation lever to
position “O”.
3. Adjust the size of the field diaphragm image
to closely match the size of the field of view,
by rotating the field diaphragm knob on the
dia pillar illuminator.
4. Adjust the brightness for the field of view by
moving the ND filters on the dia pillar
illuminator in and out of the optical path.
If color reproducibility is not required, brightness
adjustment can be performed by changing the
lamp voltage with the brightness control knob on
the left side of the microscope.
5. Move the stage to bring the observation
target into the center of the field of view.
Adjust the focus by rotating the focus knobs.
10-3
10-4
10-1
10-2
(10-2)
10-5
10-5
Objective pupil plane
perture diaphragm image
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