Validate purge
Follow the instructions below to check that there is no air left in the pump after performing a purge.
ActionStep
In the Process Picture:1
•
Click on the Injection valve and select Direct inject.
Result: The Injection valve switches to direct inject position.
Make sure that the pump flow is on.2
In the Chromatogram pane:
Check the PreC pressure curve.
3
If the PreC pressure does not stabilize within a few minutes there may be air left in the
pump. Refer to Section 8.6 Troubleshooting: Pumps, on page 387.
End the run
Click the End button in the System Control toolbar to end the run.
172 ÄKTA pure User Manual 29119969 AB
5 Operation
5.4 Prime inlets and purge pump heads
5.4.2 Sample pump