7.7.1 Calibrate the pH monitor
Maintenance interval
Calibrate the pH monitor once a day, when the pH electrode has been replaced, or if the ambient
temperature has changed by more than ± 5°C.
Required material
Use two pH calibration buffers with a difference of at least one pH unit. Preferably use a pH standard
buffer, pH 4 or pH 7, as the first calibration point, and a pH standard buffer close to the lowest or
highest pH you need to measure as your second point. Allow the buffers to equilibrate to ambient
temperature before use.
Instruction
CAUTION
pH-electrode. Handle the pH-electrode with care. The glass tip may break and cause
injury.
Follow the instructions below to calibrate the pH monitor.
ActionStep
In the Calibration dialog, select pH from the Monitor to calibrate drop-down list.1
Click the Prepare for calibration button.2
Result: The pH valve switches to the calibration position.
Enter the pH of the first pH standard buffer in the pH for buffer 1 field.3
ÄKTA pure User Manual 29119969 AB 303
7 Maintenance
7.7 Calibration procedures
7.7.1 Calibrate the pH monitor
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