6.5 Column valve C tests
Column valve C tests
The following Column valve C tests are available, for ÄKTA pure 25 and ÄKTA pure 150, respectively:
•
Column valve V9-C test
•
Column valve V9H-C test
Method description
The Column valve C test checks the functionality of Column valve C, labeled V9-C or V9H-C, and of the
integrated pressure sensors that measure pre-column and post-column pressure.
The method run takes approximately 1 minute.
Required configuration
A correctly installed Column valve C is required to run the test.
Required material
The following materials are required:
•
Syringe, 25-30 ml
•
Distilled water
•
Ref 1 tubing, see Reference capillary, on page 426.
Prepare the test
Follow the instructions below to prepare the system before method start.
ActionStep
Connect the Ref 1 tubing between Column valve C ports 1A and 1B.1
Make sure the Flow restrictor is inline.2
Direct outlet tubing W to a waste container.3
Place inlet tubing A1 into distilled water.4
Prime the inlet A1 and purge the pump. See Section 5.4.1 System pumps, on page 162.5
ÄKTA pure User Manual 29119969 AB 235
6 Performance tests
6.5 Column valve C tests
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