GE AKTApure User Manual

8.2 Troubleshooting: General Checklist
Introduction
Check the items in the following topics before starting more in-depth troubleshooting work.
System checks
•
Is the correct system selected in UNICORN System Control? For more details, see Section 5.3 Start
UNICORN and connect to system, on page 158.
•
Are the fans blowing at the back and at the right side of the system?
Monitor checks
•
Is the UV monitor set to the correct wavelength? Check the wavelengths that are used in the method.
For the predefined methods the wavelengths are set in the Method Settings phase. For more details,
see UNICORN Method Manual.
•
Is the air sensor sensitivity set to normal in UNICORN System Control:System Settings to avoid
unnecessary stops due to minor air bubbles?
Instrument communication
•
Have Node IDs been set correctly for all instrument modules? To check and change Node IDs, see
Node ID, on page 77.
Flow path checks
•
Is all tubing connected correctly? See Section 9.3 Tubing and connectors, on page420 and Section 5.2
Prepare the flow path, on page 151.
•
Is there leakage at any of the connections?
•
Is any tubing folded or twisted?
•
Is the inlet tubing correctly immersed in the buffer solution, beneath the liquid surface but not too
close to the flask bottom?
•
Have Fraction collector F9-R and Fraction collector F9-C been correctly prepared? For more details,
see relevant sections in Chapter 3 ÄKTA pure external modules, on page 87 and Chapter 4 System
configuration, on page 124.
•
Are the inlet and inline filters clean or are they generating a back pressure higher than normal? If
this is the case, change the inline and inlet filters. For more details, see Section 7.3.2 Replace the
inline filter, on page 266 and Section 7.8.8 Replace the inlet filters, on page 331.
358 ÄKTA pure User Manual 29119969 AB
8 Troubleshooting
8.2 Troubleshooting: General Checklist

GE AKTApure Specifications

General

Brand	GE
Model	AKTApure
Category	Laboratory Equipment
Language	English