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AKTApure
GE AKTApure User Manual
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Action
Step
Attach
the
column
to
the
column
holder
.
2
Connect
a
suitable
tubing
to
a
Column
valve
port
,
in
this
example
port
1A
.
3
Open
the
System
Control
module.
4
In
the
Process
Picture
:
5
•
Click
on
the
Column
.
•
Select
Column
down
flow
.
Result:
The
Column
valve
switches
to
position
1
.
174
ÄKT
A
pur
e
User
Manual
29119969
AB
5
Operation
5.5
Connect
a
column
173
175
Table of Contents
Default Chapter
3
Table of Contents
3
1 Introduction
7
This Chapter Contains the Following Sections
7
See Page
7
Important User Information
8
ÄKTA Pure Overview
10
ÄKTA Pure User Documentation
12
2 The ÄKTA Pure Instrument
14
Overview Illustrations
15
Liquid Flow Path
26
Instrument Control Panel
28
Instrument Modules
33
System Pumps
34
Mixer
38
Valves, Overview
39
Inlet Valves
40
Mixer Valve
47
Injection Valve
50
Loop Valve
53
Column Valves
56
Versatile Valve
60
Ph Valve
61
Outlet Valves
65
Pressure Monitors
68
UV Monitors
70
Conductivity Monitor
73
Flow Restrictor
75
Installation of Internal Modules
77
Accessories
80
3 ÄKTA Pure External Modules
87
External Air Sensors
88
Fraction Collector F9-C
90
Function
91
Fraction Collector F9-C Illustrations
93
Cassettes, Cassette Tray and Racks
97
Connect Tubing to the ÄKTA Pure Instrument
103
Fraction Collector F9-C
103
Fraction Collector F9-R
104
Sample Pump S9 and S9H
108
I/O-Box E9
112
Overview of the I/O-Box
113
I/O-Box E9
113
Analog Connector and Signals
115
Digital Connector and Signals
117
Connect External Equipment to the I/O-Box
119
Connection of External Modules
122
4 System Configuration
124
Configuration Overview
125
Configure Modules
131
Configuration of Inlet Valves
132
Configuration of Mixer Valves
133
Configuration of Loop Valves
134
Configuration of Column Valves
135
Configuration of Versatile Valves
137
Configuration of Ph Valves
138
Configuration of Outlet Valves
139
Configuration of UV Monitors
140
Configuration of Conductivity Monitor
141
Configuration of External Air Sensors
142
Configuration of Fraction Collectors
143
Configuration of I/O-Box
145
General System Settings
146
5 Operation
149
Before You Prepare the System
150
Prepare the Flow Path
151
Start UNICORN and Connect to System
158
Prime Inlets and Purge Pump Heads
161
System Pumps
162
Sample Pump
168
Connect a Column
173
Pressure Alarms
177
Sample Application
180
Sample Application Using Direct Injection Onto the Column
181
Sample Application Using a Superloop
183
Sample Application Using a Sample Loop
190
Fractionation
193
Prepare Fraction Collector F9-C
194
Prepare Fraction Collector F9-R
200
Fractionation Overview
207
Create a Method and Perform a Run
211
Create a Method
212
Prepare and Perform a Run
217
Monitor a Run
219
After Run Procedures
221
6 Performance Tests
225
General Performance Test Actions
226
Air Sensor a and Inlet Valve a Tests
229
Air Sensor B and Inlet Valve B Tests
231
Air Sensor S and Sample Inlet Valve Tests
233
Column Valve C Tests
235
Fraction Collector F9-C Test
237
Fraction Collector F9-R Test
240
Sample Pump Tests
242
System Test UV U9-L (Fixed)
244
System Test UV U9-M (Variable)
248
7 Maintenance
252
Maintenance Manager
253
Maintenance Program
258
Weekly Maintenance
261
Change Pump Rinsing Solution
262
Replace the Inline Filter
266
Clean the Fraction Collector F9-C Sensors
267
Monthly Maintenance
269
Semiannual Maintenance
272
Clean the UV Flow Cell
273
Replace the Ph Electrode
275
Maintenance When Required
276
Clean the Instrument Externally
277
Perform System CIP
278
Perform Column CIP
284
Clean Fraction Collector F9-C
288
Clean Fraction Collector F9-R
290
Clean Fraction Collector
290
Storage of the Ph Electrode
291
Clean the Ph Electrode
293
Clean the Pump Head Check Valves
296
Wipe off Excess Oil from the Pump Head
299
Clean the Conductivity Flow Cell
300
Calibration Procedures
302
Calibrate the Ph Monitor
303
Calibrate the Pressure Monitors
305
Calibrate the Conductivity Monitor
308
Calibrate the UV Monitors
312
Replacement Procedures
316
Replace Tubing and Connectors
317
Replace Internal Tubing in Fraction Collector F9-C
319
Replace the Mixer
322
Replace the O-Ring Inside the Mixer
323
Replace the UV Monitor U9-M Flow Cell
325
Replace the UV Monitor U9-L Flow Cell
328
Replace Flow Restrictor
330
Replace the Inlet Filters
331
Replace the Pump Head Check Valves
332
Replace Pump Piston Seals of Pump P9 or P9H
335
Replace Pump Piston Seals of Pump P9-S
344
Replace Pump Pistons
351
Replace Pump Rinsing System Tubing
352
8 Troubleshooting
355
Introduction to Troubleshooting
356
Troubleshooting: General Checklist
358
Troubleshooting: Monitors
360
Troubleshooting: Valves
375
Troubleshooting: Fraction Collector
377
Troubleshooting: Pumps
387
Troubleshooting: Other Components
394
Troubleshooting: Instrument Communication
401
Troubleshooting: Method Development
403
Error Codes
404
9 Reference Information
410
System Specifications
411
Module Specifications
413
Tubing and Connectors
420
Recommended Tubing Kits for Prepacked Columns
428
Chemical Resistance Guide
431
General Information about Biocompatibility and Chemical Resistance
432
Chemical Resistance Specifications
433
Wetted Materials
436
Predefined Methods and Phases
438
Predefined Purification Methods
439
Predefined Maintenance Methods
445
Predefined Phases
447
System Settings
449
System Settings - UV
450
System Settings - Conductivity
451
System Settings - Ph
452
System Settings - Pressure Alarms
453
System Settings - Air Sensor
455
System Settings - I/O-Box
457
System Settings - Fraction Collection
458
System Settings - Tubing and Delay Volumes
460
System Settings - Wash Settings
462
System Settings - Watch Parameters
463
System Settings - Advanced
464
System Settings - Data Collection
465
Manual Instructions
466
Manual Instructions - Pumps
467
Manual Instructions - Flow Path
471
Manual Instructions - Monitors
473
Manual Instructions - Fraction Collection
474
Manual Instructions - I/O-Box
478
Manual Instructions - Alarms
479
Manual Instructions - Wash Settings
482
Manual Instructions - Watch Parameters
483
Manual Instructions - Advanced
484
Manual Instructions - Other
487
Available Run Data
488
Available Curves
491
Injection Volumes and Peak Broadening
493
Delay Volumes
494
Component Volumes
499
Pressure Control
501
Node Ids
503
10 Ordering Information
506
Index
514
5
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GE AKTApure Specifications
General
Brand
GE
Model
AKTApure
Category
Laboratory Equipment
Language
English
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