DescriptionFlow path
The system flow is directed onto the column or column valve. Sample
can be manually injected into the loop.
Excess sample leaves the valve through waste port W1.
Manual load - Default posi-
tion of the valve
The system flow is directed through the loop and onto the column
or column valve. If a mixer valve or the sample pump is used, the
flow entering the SaP port is directed to waste port W2.
Inject
The system flow is directed to waste port W1. If a mixer valve or the
sample pump is used, the flow entering the SaP port is directed to
the column or the column valve.
System pump waste
The flow entering the SaP port is directed to the column or the col-
umn valve. This position is used with:
Direct inject
•
the sample pump, or
•
a mixer valve and System pump A, bypassing the mixer.
Flow entering the SyP port is directed to waste port W1.
The flow entering the SaP port is directed to the loop. This position
is used with:
Sample pump load
•
the sample pump, or
•
a mixer valve and System pump A, bypassing the mixer.
Excess sample leaves the valve through waste port W1. The flow
entering the SyP port is directed to the column or the column valve.
The flow entering the SaP port is directed to waste port W2. This
position is used with:
Sample pump waste
•
the sample pump, or
•
a mixer valve and System pump A.
The flow entering the SyP port is directed to the column via the loop.
Note: •
In order to avoid sample carry-over when switching techniques for loading samples, wash
the injection valve with buffer between the loading of two different samples. For example,
when switching from loading sample onto the loop to loading sample directly onto the
column with the valve in Direct inject position.
•
Make sure that the SaP port is plugged with a stop plug if neither the sample pump nor
the mixer is used.
52 ÄKTA pure User Manual 29119969 AB
2 The ÄKTA pure instrument
2.4 Instrument modules
2.4.6 Injection valve
To Next Page
Loading...
Need help?
General
