Nikon ECLIPSE Ti2-U User Manual

Chapter 5 Troubleshooting
110
5.1.4 Troubles in DIC and IMSI Microscopies
Problem Check item
A portion of the field of view is missing.
Condenser
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Turn the condenser turret to the regular position. ( ☞ 3.4.6)
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For an HNA condenser slider, move the slider to the limit position. ( ☞ 3.4.6)
Nosepiece / objective
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Push in the DIC slider until it stops. ( ☞ 3.9.2)
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Correctly attach the nosepiece. ( ☞ 7.6)
There is no contrast in
the DIC or IMSI image.
Polarizer / Analyzer
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Place the polarizer and the analyzer into the optical path. ( ☞ 3.5.1, 3.5.2, 4.3.2)
Nosepiece / objective
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Place the DIC/IMSI objective into the optical path. ( ☞ 3.9.1, 4.3.2)
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Place the DIC slider into the optical path. ( ☞ 3.9.2, 4.3.2)
Condenser module
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Place the DIC/IMSI module into the optical path ( ☞ 3.4.6, 4.3.2)
Combination of optical elements
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Confirm that the combination of the objective, the DIC slider and the condenser
module is correct. (
☞ 4.3.3)
The contrast in DIC or IMSI image is
poor.
Polarizer / Analyzer
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Turn the polarizer rotation lever until a dark cross appears. ( ☞ 3.5.1)
Combination of optical elements
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Select the combination of the optical elements suitable for the specimen. ( ☞ 4.3.3)
Specimen / stage
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Check whether plastic vessels or lids are used. ( ☞ 4.3.1)
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Use a glass slide and a cover glass free of distortion, dust, or dirt. ( ☞ 4.3.1)
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When a combination of an oil-immersing objective and a heat plate are used for
IMSI microscopy, a heat plate with a hole must be used.
The total thickness of the heat plate and the cover glass must be within the
supporting range (0.6 to 1.3 mm) of the correction collar.

Nikon ECLIPSE Ti2-U Specifications

General

Brand	Nikon
Model	ECLIPSE Ti2-U
Category	Microscope
Language	English