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ECLIPSE Ti2-U
Nikon ECLIPSE Ti2-U User Manual
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Chapter
4 Microscopy
T
echniques
79
Chapter 4
Microscopy T
echniques
Chapter
4
Microscopy T
echniques
This chapter explain
s the principles of each microscopy
, and optical elements require
d for implementing
each microscopy
, and combinations of optical elements.
98
100
Table of Contents
Default Chapter
3
Introduction
3
Table of Contents
4
Meaning of Symbols Used on the Product
9
Safety Precautions
9
Caution
10
Warning
10
WARNING and CAUTION Symbols Used in this Manual
10
Handling of this Product
19
Chapter 1 Names of Parts and Their Functions
21
System Configuration and Names of Components
22
Nomenclature and Functions
24
Dia-Illumination Section
24
Condenser Unit and Polarizer
25
Tube and Eyepiece
26
Tube Base Unit
26
Stage
27
Nosepiece
28
Objectives
28
FL Turret and Analyzer
29
Epi-Fluorescence Attachments
30
Operation Sections of the Microscope Main Body
32
Front Operation Panel
32
Right and Left Operation Panels
32
Power Switch
33
AC Adapter and Power Cord
33
Chapter 2 Microscopy
35
Diascopic Bright-Field (BF) Microscopy
36
Diascopic Phase Contrast (Ph) Microscopy
38
Diascopic DIC / IMSI Microscopy
40
Episcopic Fluorescence (Epi-FL) Microscopy
42
Diascopic Dark-Field (DF) Microscopy
44
NAMC Microscopy
46
Chapter 3 Usage of Components
49
Power-Related Operation
50
Optical Parts in the Microscope Main Body
51
Switching the Output Port
51
Switching the Magnification of the Device with the Intermediate Magnification Dial
52
Using the Dia-Illumination Section
53
Opening/Closing the Dia-Illumination Shutter
53
Using Diascopic Illumination
53
Adjusting the Dia Field Diaphragm
54
Using a Filter for Dia-Illumination
55
Using the Dia-Illumination Pillar
56
Operating the Contrast Shield (Optional)
57
Using the D-LH/LC Precentered Lamphouse
58
Using the Condenser Section
61
Selecting a Suitable Condenser
61
Elevating the Condenser
62
Adjusting the Position of the Condenser (Centering)
62
Using an Oil Immersion Condenser Lens
63
Adjusting the Diascopic Aperture Diaphragm
64
Switching the Condenser Module
65
Adjusting the Ph Module Position
67
Adjusting the NAMC Module
68
Using the ELWD-S Condenser
70
Using the Polarizer and Analyzer
71
Using the Polarizer
71
Using the Analyzer
73
Using the Tube and the Objective
74
Adjusting the Angle of the Binocular Part (er Tube)
74
Adjusting the Diopter of the Eyepiece
75
Adjusting Interpupillary Distance
76
Operating the Centering Telescope
76
Using the Tube Base Unit
77
Switching the Optical Path of the Tube Base Unit (Tube Base Unit with Port)
77
Using the Stage
78
Setting a Sample on the Manual Stage
78
Setting a Sample on the Plain Stage/Gliding Stage
79
Moving a Sample on the Stage
80
Using the Nosepiece
81
Turning the Nosepiece to Switch the Objective
81
Placing the DIC Prism into the Optical Path
81
Using the Objective
82
Adjusting the Objective with the Correction Collar
82
Using Oil Immersion Objectives
84
Using a Water Immersion Objective
85
Using an Objective with a Diaphragm
86
Using the Focusing Device
87
Focusing on the Sample
87
Using the Coarse-Focus Knob Torque Adjustment Ring
88
Using the Objective Refocusing Lever
89
Using the FL Turret
90
Switching the Filter Cube
90
Using the Shutter of the FL Turret
90
Selecting Filter Cubes
91
Using the Epi-Fluorescence Attachment
92
Adjusting the Aperture Diaphragm of the Epi-Fluorescence Attachment
92
Using the Filter Slider
93
Using the Field Diaphragm Slider
94
Switching the Optical Path of the Main Branch
96
Using a Back Port Unit (Optional)
97
Using a Centering C-Mount Adapter
98
Chapter 4 Microscopy Techniques
99
Details of Diascopic Bright-Field (BF) Microscopy
100
Principles of BF Microscopy
100
Optical Elements Required for BF Microscopy
101
Combination of Optical Elements
102
Details of Phase Contrast (Ph) Microscopy
103
Principles of Ph Microscopy
103
Optical Elements Required for Ph Microscopy
104
Combination of Optical Elements
106
Details of Diascopic DIC and IMSI Microscopies
107
Principles of DIC Microscopy
107
Optical Elements Required for DIC Microscopy
108
Combination of Optical Elements
110
Details of Episcopic Fluorescence (Epi-FL) Microscopy
114
Principles of Epi-FL Microscopy
114
Optical Elements Required for Epi-FL Microscopy
115
Combination of Optical Elements
116
Details of Diascopic Dark-Field (DF) Microscopy
117
Principles of DF Microscopy
117
Optical Elements Required for DF Microscopy
117
Combination of the Optical Elements Required for DF Microscopy
119
Details of NAMC Microscopy
120
Principles of NAMC Microscopy
120
Optical Elements Required for NAMC Microscopy
121
Combination of Optical Elements
122
Chapter 5 Troubleshooting
123
Troubleshooting for Microscopy Techniques
124
Common Microscopy Troubles
124
Troubles in BF Microscopy
127
Troubles in Ph Microscopy
129
Troubles in DIC and IMSI Microscopies
130
Troubles in Epi-FL Microscopy
131
Troubles in DF Microscopy
131
Troubles in NAMC Microscopy
132
Troubleshooting for the Units
133
Microscope Main Body
133
Problems with the Dia-Illumination Section
133
Pillar for Dia Illumination and Condenser
134
Stage
134
Nosepiece and Focusing Device
134
Epi-Fluorescence Attachment
134
Chapter 6 Maintenance and Storage of the Device
135
Cleaning the Device
136
Cleaning the Lenses
137
Cleaning Parts Other than Lenses
137
Cleaning off Immersion Oil
137
Decontaminating the Product
137
Storing the Product
138
Regular Inspection (Charged)
138
Chapter 7 Assembly of the Devices
139
Ti2-U System Configuration
141
List of Components
143
Installing the Microscope Main Body (Base)
147
Removal and Installation
147
Mounting the Tube Base Unit
148
FL Turret (Required for Epi-FL Microscopy)
149
Attaching an FL Turret
149
Mounting a Filter Cube
150
Replacing an Excitation Filter, a Barrier Filter, and a Dichroic Mirror
151
Attaching Optical Elements to the C-FL-HQ Filter Cube
155
Attaching a Nosepiece
157
Mounting a Stage
158
Mounting the Stage
158
Attaching a Stage Handle (Manual Stage Only)
159
Removing a Fixing Tool of the Stage
161
Restriction on Stage Strokes (Manual Stage Only)
162
Mounting a Diascopic Illumination Pillar and a Lamphouse
163
Mounting an Illumination Pillar
163
Attaching the Condenser Holder
164
Attaching a Fixed Filter for Dia-Illumination
165
Attaching a Filter Slider for Dia Illumination (Optional)
166
Attaching a Motorized Dia-Illumination Shutter (Optional)
167
Mounting a Lamphouse for Dia-Illumination
168
Replacing a Dia-Illumination Lamp (Precentered Lamphouse Only)
169
Attaching a Contrast Shield (Optional)
170
Attaching an Analyzer and a Polarizer (Required for DIC Microscopy)
171
Attaching an Analyzer
171
Attaching a Polarizer
172
Attaching a Condenser
173
Preparation for Attaching a Condenser
173
Attaching a Condenser Turret (System Condenser)
174
Attaching an ELWD-S Condenser
177
Attaching a DF Condenser
177
Attaching a High NA Condenser
178
Attaching the Tube and Eyepieces
180
Attaching an Epi-Fluorescence Attachment
181
Mounting an EPI-FL Module (TI2-LA-FL-2)
181
Attaching a Field Diaphragm Slider
185
Attaching a Camera Device
186
Attaching a Camera Device to the Side Port (Microscope Main Body)
186
Attaching a Camera Device to the Side Port on the Tube Base Unit
188
Attaching the Back Port Unit (Optional)
189
Connection of each Cable
190
Position of each Connector
190
Clamping Cables
191
Attaching Objectives
192
Attaching CFI60 Objectives
192
Parfocal Adjustment of Objectives Without Correction Collar
193
Attaching a Specimen Holder
195
Attaching a Specimen Holder for the Manual Stage
195
Attaching a Stage Ring
195
Moving the Microscope
196
Chapter 8 Specifications
197
Principles of Operation
198
Performance Properties
199
Physical Properties
203
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Nikon ECLIPSE Ti2-U Specifications
General
Brand
Nikon
Model
ECLIPSE Ti2-U
Category
Microscope
Language
English
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