Chapter 3 Usage of Components
64
3.10.2 Using Oil Immersion Objectives
Objectives marked with “Oil” are oil immersion
objectives.
Operation:
• When using an oil immersion objective, fill the
gap between the tip of the objective and the
specimen with oil (Nikon immersion oil). When
performing fluorescence microscopy using an
oil immersion objective for fluorescence mi-
croscopy, use low-fluorescence oil.
• After using an oil immersion objective, wipe the
oil off the tip.
Example of oil immersion objective
Immerse the objective in the oil.
1. Lower the objective.
2. Apply the minimum required amount of oil
at the tip of the objective.
Take care not to let air bubbles get mixed in. In
addition, use as little oil as possible (just
enough to fill the gap between the tip of the
objective and the specimen), and take care not
to allow the oil to adhere to other parts.
3. Place the specimen on the stage.
4. Slowly raise the objective by turning the
focus knobs, allowing the oil on the tip of
the objective to touch the bottom surface of
the specimen.
5. Check the oil for bubbles.
6. Observe the specimen while operating the
stage and the focus knobs.
7. After using an oil immersion objective, wipe
the oil off the tip.
Oil immersion
Handling of immersion oil
• If air bubbles are mixed in the immersion oil, the quality of the microscope images will decrease. When
using immersion oil, take care not to let air bubbles get mixed in.
• Oil remaining at the tip of the oil-immersion lens after the wiping or oil adhering to the tip of the dry lens
will adversely affect the image. After use, thoroughly wipe off all oil, and make sure that no oil adheres
to the tips of other objectives.
Wiping off oil
• When wiping off oil, use a lens tissue or clean cloth moistened with benzine and gently wipe off a few
times. To wipe off oil cleanly, be sure to use the same portion of the tissue or cloth only once. For a
clean finish, use absolute alcohol (ethyl alcohol or methyl alcohol) at the end.
• If petroleum benzine is unavailable, use methyl alcohol instead. However, because the cleaning powe
of methyl alcohol is weak, the user may need to wipe several times (usually three or four times).
• When wiping oil off the specimen, take due care not to damage the specimen.
Using the refocusing ring
Use the objective refocusing lever to efficiently refocus on the specimen when oil-immersion observation is
repeated.
“Oil” mark
Immersion oil
Tip of the objective
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