Chapter 7 Assembly of the Devices
172
7.16 Attaching Objectives
Attach an objective to the nosepiece.
Notes on attaching an objective
• If no stage is attached, attach one.
• Nikon recommends attaching objectives after assembling all devices.
However, remove stage rings, holders, and the like from the stage.
• To prevent objectives from impacting the stage, turn the focus knobs to lower the nosepiece to the bottom
position before attaching the objectives.
• To prevent incidence of extraneous light and intrusion of dust and solutions (which might cause failure if
spilled inside), be sure to put supplied objective screw caps on objective mount holes with no objective
attached, and supplied DIC slider slot caps on DIC slider slots without a DIC slider attached.
• To perform parfocal adjustment with an objective without correction collar, a parfocal washer set must be
attached between the objective and the nosepiece. (See §7.16.2.)
7.16.1 Attaching CFI60 Objectives
1. Screw in an objective to the objective mount
hole of the nosepiece through the stage
hole.
Screw in the objective to the nosepiece in such
a way that when turning the nosepiece clock-
wise (as seen from above), the magnification of
the objective increases.
Attaching an objective
2. Be sure to put an objective screw cap over
the unused objective mount hole of the
nosepiece.
3. To perform DIC microscopy, attach a DIC
slider.
Remove the DIC slider slot cap immediately
under the DIC objective that is attached to the
nosepiece, and insert a DIC slider suitable for
the DIC objective.
Note that the type of the DIC slider to be used
depends on the objective.
For details on the combination of objectives and
DIC sliders, see “4.4.3 Combination of Optical
Elements.”
Attaching an objective screw cap and a DIC slider
Nosepiece
Objective
Objective screw
cap
DIC slide
DIC slide
slot cap
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