Chapter 3 Usage of Components
65
CAUTION
Take due care when handling absolute alcohol or petroleum benzine, as they are highly inflammable.
Do not bring any ignition source or use the power switch near absolute alcohol or petroleum
benzine.
Checking for air bubbles
• Air bubbles can be checked for by observing the pupil plane of the objective. Remove the eyepieces,
attach the centering telescope, and focus on the pupil plane of the objective by turning the eyepiece part
of the centering telescope.
• If there are air bubbles, slightly turn the nosepiece to move the oil-immersed objective once or twice to
remove the air bubbles. If the air bubbles still remain, wipe off the oil and then apply it again.
(1) Objective pupil when the Bertrand lens
is placed into the optical path
(2) Field of view after the focus of the
Bertrand lens changed from (1)
3.10.3 Using a Water Immersion Objective
Objectives marked with “WI” are water immersion
objectives. Use water immersion objectives for
inverted microscopes.
Operation:
• When using a water immersion objective, fill the
gap between the tip of the objective and the
specimen with pure or distilled water.
• After using a water immersion objective, wipe
the water off the tip.
Example of a water immersion objective
Handling a water immersion objective
• When immersing an objective in water, avoid using tap water. There are impurities in tap water that
might adhere to the glass surface and harden when the water at the lens tip dries off. This might
cause the lens to be scratched or damaged at the time of cleaning.
• Plan Apo 60×WI (NA = 1.2) is provided with a correction collar so that the optimal aberration can be
obtained for variations of cover glass thickness. Scale display “17” indicates 0.17 mm. For precise
correction, measure the thickness of the cover glass with an instrument such as a micrometer and
match the thickness with the graduation level of the correction collar, before using the cover glass.
• After using the objective, wipe the water off the surface. Otherwise, the metallic portions might
become discolored or rusted, or the lens may lose transparency.
• After wiping the water off the surface of the objective, use absolute alcohol for a finishing wipe.
• If water dries off naturally and a smear-type stain remains, apply a small amount of neutral detergent,
gently wipe off the stain, and then use absolute alcohol for a finish wipe.
“WI” mar
Light in the upper
field of view is
shielded.
n air bubble can
be observed in the
upper field of view.
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