Chapter 2 Microscopy
26
2.6 NAMC Microscopy
1
Focus on the sample with BF
microscopy.
1.1 Perform the procedure from step
1
to step
3
in “2.1 Diascopic Bright-field (BF) Mi-
croscopy.”
Configuration of the microscope
To conduct Nikon Advanced Modulation Contrast (NAMC)
microscopy, a polarizer for NAMC/IMSI, a condenser module
(slit diaphragm) for NAMC, and an objective for NAMC are
required in addition to the configuration for BF microscopy.
Provide illumination from the side through the polarizing plate
and the slit diaphragm, and adjust the azimuth of the
modulator in the objective in order to provide contrast to the
colorless and transparent image of the sample. This causes
different light intensities for microscopy observation.
Note: Letters within [ ] correspond to those in the procedure.
2
Set up for NAMC microscopy, and adjust the
modulator.
2-1 Place the objective for NAMC into the optical
path.
[a] Nosepiece (
☞
3.9.1)
▼
2-2 Focus on the sample.
[b] Focus knob (
☞
3.11.1)
▼
2-3 Place the NAMC condenser module having
the same NAMC code as the objective into
the optical path.
[c] Condenser turret (
☞
3.4.6)
▼
2-4
Remove one of the eyepieces and attach the
centering telescope.
[d] Centering telescope (☞ 3.6.4)
▼
2-5 Check that the polarizer for NAMC/IMSI is in
the optical path.
[e] Polarizer for NAMC/IMSI (
☞
3.5.1)
▼
2-6 Turn the eyepiece section of the centering
telescope to focus on the modulator of the
objective.
[d] Centering telescope (☞ 3.6.4)
▼
2-7 Adjust the azimuth of the polarizer for
NAMC/IMSI so that two slit diaphragms can
be seen.
[e] Polarizer for NAMC/IMSI (
☞
3.5.1)
▼
2-8 Adjust the azimuth of the modulator of the
objective.
[f] Objective for NAMC (
☞
3.4.8, 4.6.2)
Contrast appears vertically to the azimuth of the
modulator.
Adjust the direction of contrast so that the specimen
can be observed clearly.
▼
2-9 Adjust the azimuth of the slit diaphragm.
[g] NAMC condenser module (
☞
3.4.8)
Adjust the azimuth so that the longitudinal direction of
the slit aperture image becomes parallel to the gray
zone in the modulator.
▼
[a]
[g]
[i]
[b]
[f]
[c]
[j]
[e]
[d]
[h]
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