Chapter 2 Microscopy
19
2.2 Diascopic Phase Contrast (Ph) Microscopy (Continued)
3
Perform Ph microscopy.
3-1 Fully open the dia aperture diaphragm.
[g] Aperture diaphragm open/close lever (
☞
3.4.5)
▼
3-2 Adjust the size of the dia field diaphragm
image.
[h] Field diaphragm dial (
☞
3.3.3)
▼
3-3 When using an objective with a correction
collar, adjust the correction collar.
[b] Objective (
☞
3.10.1)
Adjust the correction collar of the objective according
to the thickness of the prepared specimen slide or the
cover glass of the culture vessel.
▼
3-4 Move the stage and turn the focus knobs to
observe the sample.
[i] Stage handle (
☞
3.8.3)
[c] Focus knob (
☞
3.11.1)
▼
3-5 When using another objective, repeat the
procedure from step
2
.
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