Chapter 2 Microscopy
23
2.4 Episcopic Fluorescence (Epi-FL) Microscopy (Continued)
3
Adjust the epi field diaphragm.
3-1 Turn the epi field diaphragm knob until the
field diaphragm image becomes visible in the
field of view.
[k] Field diaphragm open/close lever (
☞
3.13.3)
▼
3-2 Move the epi field diaphragm image to the
center of the field of view.
[k] Field diaphragm centering screw (
☞
3.13.3)
▼
3-3 Adjust the size of the epi field diaphragm
image to closely match the size of the field of
view.
[k] Field diaphragm open/close lever (
☞
3.13.3)
▼
3-4 For a fine adjustment, change the objective to
a higher magnification objective and repeat
the procedure from step 3-1 to step 3-3.
4
Perform Epi-FL microscopy.
4-1 Change the objective to the objective for
fluorescence microscopy with a specified
magnification.
[b] Nosepiece (
☞
3.9.1)
▼
4-2 When using a liquid immersion type
objective, perform oil or water immersion.
[f] Objective (
☞
3.10.2, 3.10.3)
▼
4-3 Adjust the size of the epi field diaphragm
image and move it to the center of the field of
view.
[k] Field diaphragm open/close lever (
☞
3.13.3)
[k] Field diaphragm centering screw (
☞
3.13.3)
▼
4-4 When an objective with correction collar is
used, adjust the correction collar.
[f] Objective (
☞
3.10.1)
Adjust the correction collar of the objective according
to the thickness of the prepared specimen slide or the
cover glass of the culture vessel.
▼
4-5 Move the stage and turn the focus knobs to
observe the sample.
[o] Stage handle (
☞
3.8.3)
[p] Focus knob (
☞
3.11.1)
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