Nikon Eclipse TI-TIRF-E User Manual

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Contents
1. System Configurations and Part Names............................................................................................1-4
1.1 Overview ........................................................................................................................................ 1-4
1.1.1 TIRF Illuminator Unit and Ti-U System Overview .............................................................. 1-4
1.1.2 Motorized TIRF Illuminator Unit and Ti-E System Overview ............................................. 1-8
1.1.3 Motorized TIRF Illuminator Unit, LU4A, N-STORM, and Ti-E System Overview............. 1-12
1.1.4 TI-PAU Photo Activation Illuminator Unit and Ti-U System Overview.............................. 1-14
1.2 Optical Paths of Lasers ................................................................................................................ 1-18
1.2.1 Laser Unit......................................................................................................................... 1-18
1.2.2 Microscope Part (TIRF Illuminator Unit and Ti-U System)............................................... 1-19
1.2.3 Microscope Part (Motorized TIRF Illuminator Unit and Ti-E System) .............................. 1-20
1.2.4 Microscope Part (Motorized TIRF Illuminator Unit, LU4A, N-STORM, and Ti-E System)1-21
1.2.5 Microscope Part (Photo Activation Illuminator Unit and Ti-U System)............................. 1-22
1.3 Safety Labels and Openings ........................................................................................................ 1-23
1.3.1 Ti-U Microscope Body (with the TIRF Illuminator Unit).................................................... 1-24
1.3.2 Ti-E Microscope Body (with the Motorized TIRF Illuminator Unit) ................................... 1-25
1.3.3 Ti-U Microscope Body (with the TI-PAU Photo Activation Illuminator Unit)........................ 1-26
1.3.4 C-LU2 Two-laser Unit....................................................................................................... 1-27
1.3.5 C-LU3 Three-laser Unit.................................................................................................... 1-28
1.3.6 C-LU3EX Three-laser Unit EX ......................................................................................... 1-29
1.3.7 LU4A Four-laser Module A............................................................................................... 1-30
1.3.8 LU-LR Four-laser PS Rack .............................................................................................. 1-31
1.3.9 TI-LUSU Shutter Unit ....................................................................................................... 1-32
1.3.10 TI-LU4SU Shutter Unit LU4 ............................................................................................. 1-34
1.3.11 TI-TIRF TIRF Illuminator Unit........................................................................................... 1-36
1.3.12 TI-TIRF-E Motorized TIRF Illuminator Unit ― For Normal Microscopy
(Except N-STORM).......................................................................................................... 1-37
1.3.13 TI-TIRF-E Motorized TIRF Illuminator Unit ― For N-STORM Microscopy...................... 1-38
1.3.14 TI-PAU Photo Activation Illuminator Unit ......................................................................... 1-39
1.4 Name of Each Part....................................................................................................................... 1-40
1.4.1 TIRF Illuminator Unit and Ti-U System ............................................................................ 1-40
1.4.2 Motorized TIRF Illuminator Unit and Ti-E System............................................................ 1-45
1.4.3 Motorized TIRF Illuminator Unit, LU4A, N-STORM and Ti-E System.............................. 1-49
1.4.4 TI-PAU Photo Activation Illuminator Unit and Ti-U System.............................................. 1-54
1.4.5 Laser Unit......................................................................................................................... 1-58
1.4.6 Laser Safety Kit................................................................................................................1-62
1.4.7 Related Product ............................................................................................................... 1-64
2. Preparations........................................................................................................................................ 1-67
2.1 Check Items and Tools................................................................................................................. 1-67
2.1.1 Required Items................................................................................................................. 1-67
2.1.2 Tools................................................................................................................................. 1-68
2.2 Overview of Setup Procedure ...................................................................................................... 1-69
3. Setting Up the PC and Operation Software ..................................................................................... 1-70

Nikon Eclipse TI-TIRF-E Specifications

General

Brand	Nikon
Model	Eclipse TI-TIRF-E
Category	Laboratory Equipment
Language	English