15-2 Art: 714377-00K Rev. Date: 31-Jan-12
Before Use
i-STAT Calibration Verification solutions require different temperature
stabilization times depending on whether or not oxygen is to be measured. If
oxygen is to be measured, equilibrate the ampule to room (ambient) temperature
for 4 hours. If not, equilibrate the ampule to room (ambient) temperature for
30 minutes.
Procedure
STEP ACTION
1 Immediately before use, shake the ampule vigorously for 5 to 10
seconds to equilibrate the liquid and gas phases. To shake, hold
the ampule at the tip and bottom with forefinger and thumb to
minimize increasing the temperature of the solution. If necessary,
tap the tip of the ampule to send solution back into the bottom
section of the ampule.
2 Protect fingers with gauze, tissue or glove, or use an ampule breaker
to snap off the tip of the ampule at the neck.
3 Immediately transfer the solution from the ampule into a plain
capillary tube or plain syringe, and then immediately transfer the
solution into a cartridge.
4 Immediately seal the cartridge and insert it into an analyzer – it
is important not to expose the solution to room air since this will
alter the results.
Note: Since aqueous based solutions such as controls lack the buffering
capabilities of whole blood, the transfer process from ampule to cartridge
must be more expedient than with a patient sample.
Transfer with Capillary
Tube
Plain capillary tubes are recommended to transfer aqueous calibration
verification material from the ampule to the cartridge. When using a capillary
tube (fresh capillary tubes with sufficient fill capacity are recommended), fill
from the bottom of the ampule.
Avoid drawing solution from the surface by placing a finger over the far end
of the tube as it is inserted into the ampule.
Once the open end of the tube rests at the bottom of the ampule, uncover the
other end to allow filling by capillary action.
Transfer with Syringe
Plain syringes are recommended to transfer aqueous calibration verification
material from the ampule to the cartridge. When using a syringe (fresh 1cc or
3cc sterile syringes with 16 - 20 gauge needles are recommended), slowly draw
approximately 1mL of solution from the bottom of the ampule.
If air is trapped between the leading edge of the solution and the plunger, do
not invert the syringe to expel it; this will not affect solution near the front
of the syringe.
If air bubbles are continually drawn into the syringe, or if a bubble is trapped
near the tip of the syringe, discard the ampule and syringe and use a fresh
ampule and syringe.
Expel one or two drops from the syringe before filling the cartridge.
i-STAT CALIBRATION VERIFICATION SET
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