Rev. Date: 03-Aug-12 Art: 714184-00M
LACTATE/LAC
Lactate is measured amperometrically. The enzyme lactate oxidase, immobilized in the lactate biosensor,
selectively converts lactate to pyruvate and hydrogen peroxide (H
2
O
2
). The liberated hydrogen peroxide
is oxidized at a platinum electrode to produce a current which is proportional to the sample lactate
concentration.
Lactate Oxidase
L-Lactate + O
2
Pyruvate + H
2
O
2
Platinum electrode
H
2
O
2
2H
+
+ O
2
+ 2e
-
See below for information on factors affecting results. Certain substances, such as drugs, may affect analyte
levels in vivo.
1
If results appear inconsistent with the clinical assessment, the patient sample should be retested using
another cartridge.
Intended Use
The test for lactate, as part of the i-STAT System, is intended for use in the in vitro quantification of lactate in
arterial, venous, or capillary whole blood.
The i-STAT lactate test is useful for (1) the diagnosis and treatment of lactic acidosis in conjunction with
measurements of blood acid/base status, (2) monitoring tissue hypoxia and strenuous physical exertion,
and (3) diagnosis of hyperlactatemia.
Contents
Each i-STAT cartridge contains one reference electrode (when potentiometric sensors are included in the
cartridge configuration), sensors for the measurement of specific analytes, and a buffered aqueous calibrant
solution that contains known concentrations of analytes and preservatives. For cartridges that contain a
sensor for the measurement of lactate, a list of reactive ingredients is indicated below:
Reactive Ingredient Biological Source
Lactate N/A
Lactate Oxidase Aerococcus viridans
Metrological Traceability
The i-STAT System test for lactate measures L-lactate amount-of-substance concentration in the plasma
fraction of arterial, venous, or capillary whole blood (dimension mmol L
-1
) for in vitro diagnostic use.
Presently, no international conventional reference measurement procedure or international conventional
calibrator for lactate is available. Lactate values assigned to i-STAT’s controls and calibration verification
materials are traceable to i-STAT’s working calibrator prepared from sodium L-lactate (Sigma-Aldrich
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