Roche Diagnostics
E-12 Operator’s Manual · Version 3.1
12 ISE description Cedex Bio System
Basic operation
Basic operation
Operation of the ISE unit is integrated in the operation of the main instrument.
The following sections contain some ISE specific information on operation-related
issues.
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For general information on operating the main instrument, see the corresponding
sections in Chapter 2 Introduction to the instrument and Chapter 5 Daily operation.
For specific ISE tasks, see Chapter 13 ISE operation.
Samples
The sample tubes are placed on the sample area of the main instrument.
Calibration
The ISE unit requires frequent calibration to ensure the accuracy of the test results
obtained.
Main calibration The electrodes are calibrated with a two-point calibration using ISE Solution 1 and 2.
The main calibration is typically performed during the Prepare phase.
One-point calibration The electrodes are calibrated after each ISE measurement using the on-board ISE
Calibrator indirect/urine. This calibration is an integral part of each ISE
measurement and is performed automatically.
ISE Standby
If there is no measurement for more than three minutes, the ISE unit switches to
Standby status. In this status, ISE Calibrator indirect/urine and ISE Reference
Solution are pumped into the measuring channel and moved a short distance at
regular intervals. Also, every two hours the ISE tubing is primed with ISE Calibrator
indirect/urine and ISE Reference Solution. This is done to prevent the following
problems:
o Flow of ISE Reference Solution backwards into the measuring channel, which can
damage the electrodes because of the high ion concentration of the ISE Reference
Solution.
o Crystallization of salts in the tubing, causing blockages.
The ISE unit has a separate power supply. Therefore, the Standby status is
maintained even if the main instrument is switched off.
Damage to electrodes and possible tubing blockage
o Do not unplug or switch off the ISE power supply. Periodic flow of solutions must be
performed at all times.
o If you intend not to use the ISE unit for more than one week, you should deactivate it.
This saves ISE fluids, reduces wear and tear of the tubing, and lowers the likelihood of
malfunctions and errors occurring. (See Deactivating the ISE unit on page E-64.)
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