Roche Diagnostics
Operatorโs Manual ยท Version 3.1 E-73
Cedex Bio System 15 ISE troubleshooting
List of ISE flags
Calc Error
Meaning Calculation error.
o General calculation error.
o Slope or nonlinear standard curve cannot be calculated due to a calibration error.
Message ID 16
Priority 13
Possible cause o Calibrator outdated or deteriorated.
o Misplacement of the calibrator tubes.
Recommended actions o Repeat calibration with fresh calibrators.
o If the calibrators were incorrectly positioned, replace the calibrators and repeat
the calibration.
Ise Unstab
Meaning The signal from the electrode(s) was not stable during the measurement.
Message ID 18
Priority 6
Possible cause o There are air bubbles in the measuring segment.
o The concentration of sample is too low (for example, dialyzed samples).
o The ISE Reference Solution tubing is blocked or leaking.
o The pH of the sample is too low (for example, pH < 5.5).
o One or more O-rings are missing or damaged.
o There are salt deposits or there is an obstruction at the adapter of the ISE
Reference Solution bottle.
o The electrodes have not been serviced.
o The expiration date of the electrode has expired or the electrode is damaged.
o The electrodes and/or the electrode block is not dry.
o The preamplifier is defective.
Recommended actions 1. Perform the maintenance action Electrode Service and repeat the measurement.
2. Repeat the measurement with a suitable sample (where pH > 5.5 and sample
concentration within the test range) to establish that the ISE module is OK.
3. Check the position and condition of the O-rings and replace if necessary.
4. Check the adapter of the ISE Reference Solution bottle for salt deposits and
obstruction.