Roche Diagnostics
Operatorโs Manual ยท Version 3.4 C-9
Cedex Bio analyzer 8 Data analysis methods
Photometric measurements
Low Activity check This check is used to identify zero pipetting of sample and reagents.
With ascending reaction curve:
With descending reaction curve:
The Low Act flag and the value of the lower test range limit are displayed in the result
list if the value exceeds the lower test limit.
Rate
This section explains the rate calculations and the associated checks.
Absorbance at the time of the low activity first point (test parameter).
Absorbance at the time of the low activity last point (test parameter).
Low activity limit (test parameter).
A
cuv
last()A
cuv
first()HLLโฅโ
A
cuv
first()A
cuv
last()HLLโฅโ
Calibration Control Sample
Calculation model Endpoint
Absorbance difference lll
Endpoint Unstable check lll
Calculation model Kinetic
Limits of kinetic range lll
Absorbance difference per minute lll
Nonlinear check lll
Kinetic readings check lll
Reaction direction
lll
Antigen Excess check
ll
Test range check (level rate)
ll
Calculation of mean rate
l
Table 2 Calculation and checks for rate determination