Roche Diagnostics
C-10
Operatorโs Manual ยท Version 3.4
8 Data analysis methods Cedex Bio analyzer
Photometric measurements
Calculation model Endpoint
Calculation of absorbance
difference
The calculation of the absorbance difference is carried out for calibrators, controls,
and samples.
Two points are used to determine the rate (ฮA) of the reaction. The rate is used to
calculate the concentration of the sample, based on the kind of calibration mode
used.
Endpoint Unstable check This check applies only to the calculation model Endpoint. It is carried out to
determine if the last five measuring points used for determinations are stable.
The EP Unstab flag is displayed if the absorbance difference is not within the
specified range.
Rate in ฮA
Absorbance at the last calculation point (last calculation point, test
parameter). See the Instructions for Use for the relevant Cedex Bio
test.
Absorbance at the fist calculation point (first calculation point, test
parameter). See the Instructions for Use for the relevant Cedex Bio
test.
A (last)
Absorbance (A)
Time (T)
A (first)
T
0
T
n
ฮA
Highest absorbance obtained from the last five measuring points.
Lowest absorbance obtained from the last five measuring points.
Endpoint unstable limit in absorbance (dAbs. test parameter).