Roche Diagnostics
Operatorโs Manual ยท Version 3.4 C-11
Cedex Bio analyzer 8 Data analysis methods
Photometric measurements
Calculation model Kinetic
The calculation model Kinetics is applied to calibrations, quality controls and
samples. A linear regression analysis is carried out between the first and last points as
specified in the Cedex Bio Instructions for Use. The slope of regression line is the
rate (ฮA per minute) of the reaction. The rate is used to calculate the concentration of
the sample based on the kind of calibration mode applied on the calibration curve.
Limits of kinetic range With the Kinetic calculation model, an inspection of the reaction is carried out to
determine if the beginning and end of the kinetic range is within the defined
absorbance range. If this is not the case the measurement range is reduced
accordingly.
Determining the measurement range used for calculating the kinetics:
The first and last absorbance values ( , ) in the kinetic range are used as
the first and last measuring points ( , ) when calculating the
kinetics. All points must fulfill the following conditions:
Absorbance of the lower reaction limit (test parameter).
Minimum value = 0.0.
Absorbance of each point in the kinetic range.
Absorbance of the upper reaction limit (test parameter).
Maximum value = 2.0.
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